Recombinant Human MIF

Recombinant Human MIF

• Description: Macrophage Migration Inhibitory Factor (MIF) is a proinflammatory cytokine. MIF is involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
• CAS Number: 9000-83-3
• Gene Name: MIF
• Gene Aliases: Glycosylation-inhibiting factor, GIF, L-dopachrome isomerase, L-dopachrome tautomerase, EC=5.3.3.12, Phenylpyruvate tautomerase, GLIF, MMIF, MIF
  Recombinant Human Macrophage Migration Inhibitory Factor (MIF)
• Gene ID: 4282
• Accession Number: P14174
• Organism: Human
• Source: E. coli
• Concentration: < 1.0 EU/μg of recombinant protein as determined by the LAL method.
• Purity: >95% as determined by SDS-PAGE
• Solubility: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Function: The activity was determined by the ability to bind recombinant human CD74 in functional ELISA.
• Molecular Weight: 15
• Shipping Conditions: Ambient Temperature
• Storage Conditions: The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
• Appearance: Lyophilized Powder
• Formulation: Recombinant human MIF was lyophilized from a 0.2 μm filtered solution PBS pH 7.4