Recombinant Human TNF

• Description:

  Tumor Necrosis Factor is secreted by macrophages, monocytes, neutrophils, T cells, natural killer cells following their stimulation by bacterial lipopolysaccharides. Cells expressing CD4 secrete TNF-alpha while CD8(+) cells secrete little or no TNF-alpha. Stimulated peripheral neutrophilic granulocytes but also unstimulated cells and also a number of transformed cell lines, astrocytes, microglial cells, smooth muscle cells, and fibroblasts also secrete TNF. Human milk also contains this factor. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL-2, GM-CSF, SP, Bradykinin, immune complexes, inhibitors of cyclooxygenase, and PAF (platelet activating factor). Human TNF-alpha is a non-glycosylated protein of 17 kDa and a length of 157 amino acids. Murine TNF-alpha is N-glycosylated. Homology with TNF-beta is approximately 30%. TNF-alpha forms dimers and trimers.

• Gene Name:

  TNF

• Gene Aliases:

  Tumor necrosis factor, Cachectin, TNF, Tumor necrosis factor ligand superfamily member 2, TNF-a
  Recombinant Human TNF-alpha (TNFA)

• Gene ID:

  7124

• Accession Number:

  P01375

• Organism:

  Human

• Source:

  CHO cells

• Concentration:

  <1.0 EU/μg of recombinant protein as determined by the LAL method.

• Purity:

  >95% as determined by SDS-PAGE

• Solubility:

  A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

• Function:

  The ED(50) was determined cytolysis of murine L929 cells in the presence of Actinomycin D is ≤ 0.02 ng/mL, corresponding to a specific activity of > 5.0 x 10^7 units/mg.

• Molecular Weight:

  18

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

• Appearance:

  Lyophilized Powder

• Formulation:

  TNF-a was lyophilized from a 0.2μm filtered concentrated (1.0 mg/mL) solution in PBS, pH 7.2.

• CAS Number:

  9000-83-3