Recombinant Rat TNF

Recombinant Rat TNF

• Description: TNF is secreted by macrophages, monocytes, neutrophils, T cells, natural killer cells following their stimulation by bacterial lipopolysaccharides. Cells expressing CD4 secrete TNF-alpha while CD8(+) cells secrete little or no TNF-alpha. Stimulated peripheral neutrophilic granulocytes but also unstimulated cells and also a number of transformed cell lines, astrocytes, microglial cells, smooth muscle cells, and fibroblasts also secrete TNF. Human milk also contains this factor. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL-2, GM-CSF, SP, Bradykinin, Immune complexes, inhibitors of cyclooxygenase and PAF (platelet activating factor). Human TNF-alpha is a non-glycosylated protein of 17 kDa and a length of 157 amino acids. Murine TNF-alpha is N-glycosylated. Homology with TNF-beta is approximately 30%. TNF-alpha forms dimers and trimers.
• CAS Number: 9000-83-3
• Gene Name: TNF
• Gene Aliases: TNF-alpha, Cachectin, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tnfa, Tnfsf2, Tumor necrosis factor
  Recombinant Rat Tumor Necrosis Factor Alpha (TNF)
• Gene ID: 24835
• Accession Number: P16599
• Organism: Rat
• Source: E. coli
• Concentration: <1.0 EU/μg of recombinant protein as determined by the LAL method
• Purity: >95% as determined by SDS-PAGE
• Solubility: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
• Function: The ED50 as determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20-40 ng/mL
• Molecular Weight: 17
• Shipping Conditions: Ambient Temperature
• Storage Conditions: The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
• Appearance: Lyophilized Powder
• Formulation: Lyophilized from a 0.2 μm filtered PBS solution (pH 7.0)