Recombinant Human TGF Beta-1 (TGFB1)

Recombinant Human TGF Beta-1 (TGFB1)

• Description: TGFB1 is the prototype member of the TGF-beta super-family that includes Inhibins, Activin A, MIS (Müllerian inhibiting substance), BMP (bone morphogenetic proteins), dpp (decapentaplegic) and Vg-1. TGFB1 is the most potent known growth inhibitor for normal and transformed epithelial cells, endothelial cells, fibroblasts, neuronal cells, lymphoid cells and other hematopoietic cell types. TGF-beta inhibits the proliferation of T lymphocytes by down-regulating predominantly IL2 mediated proliferative signals and also inhibits the growth of natural killer cells. Recombinant human TGFB1 is a disulfide linked homodimer protein that migrates at approximately 26kDa under non-reducing conditions.
• CAS Number: 9000-83-3
• Gene Name: TGFB1
• Gene Aliases: Transforming growth factor beta-1, TGF-beta-1, Differentiation inhibiting factor, Latency-associated peptide, LAP
• Gene ID: 7040
• Accession Number: P01137
• Organism: Human
• Source: HEK293 cells
• Concentration: <1.0 EU/μg of recombinant protein as determined by the LAL method
• Purity: >95% as determined by SDS-PAGE
• Solubility: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Function: The ED50 as determined by the ability to inhibit the proliferation of mink lung epithelial cells was found to be in the range of 0.05-0.1 ng/ml
• Molecular Weight: 26
• Shipping Conditions: Ambient Temperature
• Storage Conditions: Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months. Avoid repeated freeze/thaw cycles.
• Appearance: Lyophilized Powder
• Formulation: Lyophilized from 0.2 μm filtered PBS solution pH 7.4