Anti-Alpha-1-Antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) (AAT/1378), CF405S conjugate

• Description:

  This antibody recognizes a protein of 54 kDa, which is identified as Alpha-1 antitrypsin (AAT). AAT is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Alpha-1 antitrypisin; SERPINA1; Alpha-1 protease inhibitor; Alpha-1 antiproteinase; AAT; Serpin A1

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  SERPINA1

• Gene ID:

  5265

• NCBI Gene ID:

  525557

• UniProt:

  P01009

• Cellular Locus:

  Secreted (extracellular)

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant human Alpha-1-Antitrypsin (AAT) protein fragment (exact sequence is proprietary)

• Target Antigen:

  Alpha-1 Antitrypsin

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  AAT/1378

• Conjugation:

  CF405S

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  IHC, FFPE (verified) | WB (verified)

• Validated Applications:

  IHC, FFPE, WB

• Field of Research:

  Cancer, Inflammation

• Positive Control:

  HepG2 Cells. Tonsil, Hepatocellular Carcinoma or Histiocytoma.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  54 kDa

• Additionnal Information:

  Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Immunofluorescence 0.5-1 ug/mL Western blotting 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years