Anti-Cycloxygenase-2 (COX-2)(COX2/1941), CF740 conjugate

• Description:

  Prostaglandins are a diverse group of autocrine and paracrine hormones that mediate many cellular and physiologic processes. Prostaglandin H2 (PGH2) is an intermediate molecule in formation of the prostaglandins. Cyclooxygenase-1 (Cox-1) and cyclooxygenase-2 (Cox-2) are prostaglandin synthases that catalyze the formation of PGH2 from arachidonic acid (AA). Cox-1 and Cox-2 are isozymes of prostaglandin-endoperoxidase synthase (PTGS). Cox-1 is constitutively expressed in most tissues and is thought to serve in general housekeeping functions. Cox-2 is efficiently induced in migratory cells responding to pro-inflammatory stimuli and is considered to be an important mediator of inflammation. Both enzymes are targets for the nonsteroidal therapeutic anti-inflammatory drugs, NSAIDs. COX2 expression is significantly increased in 85-90% of human colorectal adenocarcinomas whereas levels of COX-1 are not changed. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase; GRIPGHS; Macrophage activation-associated marker protein P71/73; PES-2; PGG/HS; PGHS-2; Prostaglandin endoperoxide synthase 2; Prostaglandin endoperoxide synthase 2 (PTGS2); TIS10

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  PTGS2

• Gene ID:

  5743

• NCBI Gene ID:

  196384

• UniProt:

  P35354

• Cellular Locus:

  Cytoplasmic

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant human COX2 protein fragment (around aa 442-572) (exact sequence is proprietary)

• Target Antigen:

  Cycloxygenase-2

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  COX2/1941

• Conjugation:

  CF740

• Source:

  Animal

• Positive Control:

  Colon or Lung Carcinoma. HepG2 cells.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% rBSA, 0.05% azide

• Molecular Weight:

  70-72 kDa

• Additionnal Information:

  For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry

• References & Citations:

  Wang, D, et al. Oncogene. 2010 Feb 11; 29(6): 781-78

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years