Anti-Cycloxygenase-2 (COX-2)(COX2/1941)

Anti-Cycloxygenase-2 (COX-2)(COX2/1941)

• Description: Prostaglandins are a diverse group of autocrine and paracrine hormones that mediate many cellular and physiologic processes. Prostaglandin H2 (PGH2) is an intermediate molecule in formation of the prostaglandins. Cyclooxygenase-1 (Cox-1) and cyclooxygenase-2 (Cox-2) are prostaglandin synthases that catalyze the formation of PGH2 from arachidonic acid (AA). Cox-1 and Cox-2 are isozymes of prostaglandin-endoperoxidase synthase (PTGS). Cox-1 is constitutively expressed in most tissues and is thought to serve in general housekeeping functions. Cox-2 is efficiently induced in migratory cells responding to pro-inflammatory stimuli and is considered to be an important mediator of inflammation. Both enzymes are targets for the nonsteroidal therapeutic anti-inflammatory drugs, NSAIDs. COX2 expression is significantly increased in 85-90% of human colorectal adenocarcinomas whereas levels of COX-1 are not changed. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase; GRIPGHS; Macrophage activation-associated marker protein P71/73; PES-2; PGG/HS; PGHS-2; Prostaglandin endoperoxide synthase 2; Prostaglandin endoperoxide synthase 2 (PTGS2); TIS10
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: PTGS2
• Gene ID: 5743
• NCBI Gene ID: 196384
• UniProt: P35354
• Cellular Locus: Cytoplasmic
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant human COX2 protein fragment (around aa 442-572) (exact sequence is proprietary)
• Target Antigen: Cycloxygenase-2
• Clonality: Monoclonal
• Isotype: IgG2b κ
• Clone: COX2/1941
• Conjugation: Purified, BSA-free
• Source: Animal
• Positive Control: Colon or Lung Carcinoma. HepG2 cells.
• Concentration: 1 mg/mL
• Buffer: PBS, no BSA, no azide
• Molecular Weight: 70-72 kDa
• Additionnal Information: For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
• References & Citations: Wang, D, et al. Oncogene. 2010 Feb 11; 29(6): 781-78
• Shipping Conditions: Room temperature
• Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years