Anti-UGT1A9 (UDP glucuronosyltransferase 1 family, polypeptide A9)(UGT1A9/1229)

• Description:

  This antibody recognizes a protein of about 60 kDa, which is identified as human UGT1A9. It does not cross-react with the other UGT1A isoforms including UGT1A7, UGT1A8, and UGT1A10 and shows a high degree of specificity. UGT1A9 is a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This MAb binds to human hepatocytes and the majority of human hepatocellular carcinomas (HCC s). In frozen sections, it stains hepatic cells and may be used as a marker of the liver. This MAb also binds to cell preparations of hepatocellular carcinoma biopsies or cell lines.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  GNT1; HLUGP4; lugP4; UDP-glucuronosyltransferase 1-I; UDP-glucuronosyltransferase 1A9 (UGT1A9); UDPGT 1-9; UDPGT; UGT-1I; UGT1*9; UGT1-09; UGT1.9; Ugt1a9; UGT1I

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  UGT1A9

• Gene ID:

  54600

• NCBI Gene ID:

  554822

• UniProt:

  O60656

• Cellular Locus:

  Endoplasmic reticulum|Microsomes

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  A synthetic peptide specific to human UGT1A9 protein

• Target Antigen:

  UGT1A9

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  UGT1A9/1229

• Conjugation:

  Purified, BSA-free

• Disease:

  Tumor

• Source:

  Animal

• Field of Research:

  Metabolism

• Positive Control:

  HepG2 cells. Liver or Hepatocellular Carcinoma (HCC)

• Concentration:

  1 mg/mL

• Buffer:

  PBS, no BSA, no azide

• Molecular Weight:

  60 kDa

• Additionnal Information:

  For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry

• References & Citations:

  Shingo Oda, Miki Nakajima, Masahiko Hatakeyama, Tatsuki Fukami, and Tsuyoshi Yokoi. Preparation of a Specific Monoclonal Antibody against Human UDP-Glucuronosyltransferase (UGT) 1A9 and Evaluation of UGT1A9 Protein Levels in Human Tissues. Drug Metabolism and Disposition, 40:1620-1627, 2012

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years