Anti-UGT1A9 (UDP glucuronosyltransferase 1 family, polypeptide A9)(UGT1A9/1229)

Anti-UGT1A9 (UDP glucuronosyltransferase 1 family, polypeptide A9)(UGT1A9/1229)

• Description: This antibody recognizes a protein of about 60 kDa, which is identified as human UGT1A9. It does not cross-react with the other UGT1A isoforms including UGT1A7, UGT1A8, and UGT1A10 and shows a high degree of specificity. UGT1A9 is a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This MAb binds to human hepatocytes and the majority of human hepatocellular carcinomas (HCC s). In frozen sections, it stains hepatic cells and may be used as a marker of the liver. This MAb also binds to cell preparations of hepatocellular carcinoma biopsies or cell lines.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: GNT1; HLUGP4; lugP4; UDP-glucuronosyltransferase 1-I; UDP-glucuronosyltransferase 1A9 (UGT1A9); UDPGT 1-9; UDPGT; UGT-1I; UGT1*9; UGT1-09; UGT1.9; Ugt1a9; UGT1I
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: UGT1A9
• Gene ID: 54600
• NCBI Gene ID: 554822
• UniProt: O60656
• Cellular Locus: Endoplasmic reticulum|Microsomes
• Host: Mouse
• Species Reactivity: Human
• Immunogen: A synthetic peptide specific to human UGT1A9 protein
• Target Antigen: UGT1A9
• Clonality: Monoclonal
• Isotype: IgG2b κ
• Clone: UGT1A9/1229
• Conjugation: Purified, with BSA
• Disease: Tumor
• Source: Animal
• Field of Research: Metabolism
• Positive Control: HepG2 cells. Liver or Hepatocellular Carcinoma (HCC)
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: 60 kDa
• Additionnal Information: For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
• References & Citations: Shingo Oda, Miki Nakajima, Masahiko Hatakeyama, Tatsuki Fukami, and Tsuyoshi Yokoi. Preparation of a Specific Monoclonal Antibody against Human UDP-Glucuronosyltransferase (UGT) 1A9 and Evaluation of UGT1A9 Protein Levels in Human Tissues. Drug Metabolism and Disposition, 40:1620-1627, 2012
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years