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MyoD1 (5.8A), 0.2mg/mL

CAT: 0037-BNUB0191-500Size: 1x 500 µLDry Ice: NoHazardous: No
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Description
Recognizes a phosphor-protein of 45 kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
CAS Number
9007-83-4
Synonyms
BHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
MYOD1
Gene ID
4654
NCBI Gene ID
181768
UniProt
P15172
Swiss Prot
P15172
Host
Mouse
Species Reactivity
Chicken, Human, Mouse, Rat
Reactivity
MyoD1
Immunogen
Recombinant mouse MyoD1 protein
Target Antigen
MyoD1
Clonality
Monoclonal
Clone
5.8A
Type
Primary
Source
Animal
Origin
Animal
Field of Research
Cancer, Developmental biology
Positive Control
Rhabdomyosarcoma
Stability
2 years
Concentration
0.2 mg/mL
Buffer
PBS, 0.05% BSA, 0.05% azide
Molecular Weight
45 kDa
References & Citations
Thulasi R et. al. Cell Growth and Differentiation, 1996, 7(4):531-41. | Wesche WA et. al. American Journal of Surgical Pathology, 1995, 19(3):261-9. | Parham DM et. al. Acta Neuropathologica, 1994, 87:605-11. |
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG1, kappa
Species
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification
PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)
Conjugate
Purified, with BSA

UniProtKB · P15172

Myoblast determination protein 1

MYOD1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P15172
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MYOD1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
O75321
Protein keywords

PTM

AcetylationMethylationPhosphoproteinUbl conjugation

Molecular function

ActivatorDevelopmental proteinDNA-binding

Biological process

DifferentiationMyogenesisTranscriptionTranscription regulation

Disease

Disease variant

Cellular component

Nucleus

Technical term

Proteomics identificationReference proteome
Product Datasheet
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