Sheep anti Mouse Dyrk (Downs Syndrome critical region)

Sheep anti Mouse Dyrk (Downs Syndrome critical region)

• Background: Dyrk, the vertebrate of Drosophilia minibrain, is a dual-specificity kinase predominately expressed in the central nervous system. The Human clone has been isolated from the Downs' syndrome critical region and it is potentially implicated in the neuropathology of the disease. Main features of the protein include an N-terminal nuclear translocation signal, a putative leucine zipper domain, a core kinase domain with some similarity to kinases involved in cell cycle regulation and a C-terminal PEST sequence. The Dyrk kinase can be phosphorylated on tyrosine residues, leading to an active kinase that can phosphorylated itself or exogenous substrates on both tyrosine and serine/threonine residues. Dyrk can also multimerize and translocate to the nucleus. Present studies on Dyrk suggest a potential role for this kinase in the exit from the cell cycle and the beginning of neuronal differentiation.
• Synonyms: Dual Specificity (Tyrosine and serine/threonine kinase) Kinase
• CAS Number: 9007-83-4
• UniProt: Q61214
• Host: Sheep
• Species Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Conjugation: Unconjugated
• Type: Polyclonal Antibody
• Source: Sheep were immunized with a recombinant protein corresponding to amino acids 486 to 763 of the mouse Dyrk1A protein.
• Applications: WB, IP, IHC
• Field of Research: Signal Transduction
• Purification Method: Ammonium Sulfate Precipitation
• Assay Principle: Detects Dyrk by immunofluorescence at 1 to 10 µg/mL. Detects Dyrk by Western blot at 1 µg/mL. Can also be used for immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.
• Stability: See expiration date on vial
• Concentration: See vial for Concentration
• Form: Provided as solution in phosphate buffered saline with 0,08% sodium azide
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• References & Citations: 1. Kentrup, H., et al., Dyrk, a dual specificity protein kinase with unique structural features whose activity is dependent on tyrosine residues between subdomains VII and VIII. J. Biol. Chem. 1996, 271, 3488-3495._x000D_ 2. Song, W.-J., et al., Isolation of Human and murine homologues of the Drosophilia minibrain gene: Human homologue maps to 21q22.2 in the Down syndrome critical region. Genomics 1996, 38, 331-339._x000D_ 3. Becker, W. , Joost, H.G. Structural and functional characteristics of Dyrk, a novel subfamily of protein kinases with dual specificity. Prog. Nucleic Acid Res. Mol. Biol. 1999, 62, 1-17.
• Shipping Conditions: Ambient Temperature, freeze upon arrival
• Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
• Functional Analysis: WB