Anti-MHC II (HLA-DRA)(169-1B5.2), CF647 conjugate

Anti-MHC II (HLA-DRA)(169-1B5.2), CF647 conjugate

• Description: This antibody detects a monomorphic general framework determinant of HLA-DR Class II antigen. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4 T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Ia3; Ia-3; H-2Ea; H2-Ea; AI323765; I-Ealpha; E-alpha-f; MHC-H2-Ea
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: HLA-DRA
• Gene ID: 3122
• NCBI Gene ID: 520048
• UniProt: P01903
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Cat, Cow, Human
• Immunogen: Human peripheral blood leukocytes
• Target Antigen: MHC-II
• Clonality: Monoclonal
• Isotype: IgG2b κ
• Clone: 169-1B5.2
• Conjugation: CF647
• Source: Animal
• Applications: Flow (verified) | IF (published)
• Validated Applications: FC, IF
• Field of Research: Immunology, MHC antigens
• Positive Control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 36 kDa (? chain) and 27 kDa (? chain)
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Nature Microbiol (2019) 4: 2035-2038. (IF)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years