Anti-MHC II (HLA-DRA)(169-1B5.2), CF568 conjugate

• Description:

  This antibody detects a monomorphic general framework determinant of HLA-DR Class II antigen. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4 T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Ia3; Ia-3; H-2Ea; H2-Ea; AI323765; I-Ealpha; E-alpha-f; MHC-H2-Ea

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  HLA-DRA

• Gene ID:

  3122

• NCBI Gene ID:

  520048

• UniProt:

  P01903

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Cat, Cow, Human

• Immunogen:

  Human peripheral blood leukocytes

• Target Antigen:

  MHC-II

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  169-1B5.2

• Conjugation:

  CF568

• Source:

  Animal

• Applications:

  Flow (verified) | IF (published)

• Validated Applications:

  FC, IF

• Field of Research:

  Immunology, MHC antigens

• Positive Control:

  Ramos, Daudi or HuT78 cells. Tonsil or lymph node

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  36 kDa (? chain) and 27 kDa (? chain)

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Nature Microbiol (2019) 4: 2035-2038. (IF)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years