Recombinant 2019-nCoV Guanine-N7_meth (N-6His)

Recombinant 2019-nCoV Guanine-N7_meth (N-6His)

• Background: The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.
• Description: Recombinant 2019-nCoV Guanine-N7 methyltransferase is produced by our E.coli expression system and the target gene encoding Ala1-Gln527 is expressed with a 6His tag at the N-terminus.
• Synonyms: SARS-CoV 2 nsp14; SARS-CoV 2 ExoN; Guanine-N7 methyltransferase
• CAS Number: 9000-83-3
• UniProt: YP_009725309.1
• Purity: Greater than 85% as determined by reducing SDS-PAGE.
• Molecular Weight: 62.9 KDa
• Shipping Conditions: The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
• Storage Conditions: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.