Recombinant 2019-nCoV Nucleocapsid Protein

Recombinant 2019-nCoV Nucleocapsid Protein

• Background: Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
• Description: Recombinant SARS-CoV-2 Nucleocapsid Protein is produced by our E.coli expression system and the target gene encoding Met1-Ala419 is expressed with a 6His tag at the N-terminus.
• Synonyms: 2019-nCoV coronavirus NP Protein; 2019-nCoV np Protein; 2019-nCoV novel coronavirus Nucleoprotein Protein
• CAS Number: 9000-83-3
• UniProt: QHD43423.2
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Bioactivity: Immobilized 2019-nCoV Nucleocapsid Protein-His(Cat#DRA31)at 2μg/ml (100μl/well) can bind 2019-nCoV NP Antibody (6G9)(Cat#DA027). The ED50 of 2019-nCoV NP Antibody (6G9)(Cat#DA027) is 5-30 ng/ml.
• Molecular Weight: 49.4kDa
• Shipping Conditions: The product is shipped on dry ice pack. Upon receipt, store it immediately at the temperature listed below.
• Storage Conditions: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.