Anti-Fibronectin(TV-1)

• Description:

  Fibronectin is a soluble dimeric glycoprotein of 440 kDa, which is present in cells, extracellular matrix, and blood. This MAb reacts with the cellular as well as plasma form of fibronectin. Reportedly, after iv administration, this MAb localizes to tumor vessels where it binds to the underlying basement. The epitope recognized by this antibody is not accessible to the circulating MAb in normal tissues, indicating that it can be used to specifically target tumor vessels in vivo. TV-1 is reportedly useful for delivering vasoactive agents to tumors to induce increased vascular permeability or blood flow prior to treatment with chemotherapeutic drugs or MAbs.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Cold insoluble globulin (CIG); FINC; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor (MSF); Ugl-Y3

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  FN1

• Gene ID:

  2335

• NCBI Gene ID:

  203717

• UniProt:

  P02751

• Cellular Locus:

  Extracellular matrix

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse, Pig, Rat

• Immunogen:

  T-cell lymphoma biopsy

• Target Antigen:

  Fibronectin

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  TV-1

• Conjugation:

  Purified, BSA-free

• Source:

  Animal

• Applications:

  Flow, surface (published) | IHC, frozen (published) | IHC, FFPE (verified) | WB (published)

• Validated Applications:

  FC, IHC, IHC, FFPE, WB

• Field of Research:

  Cell adhesion, Extracellular matrix

• Positive Control:

  SW156 cells or Kidney

• Concentration:

  1 mg/mL

• Buffer:

  PBS, no BSA, no azide

• Molecular Weight:

  220 kDa (monomer); 440 kDa (dimer)

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Cancer Res (1995) 55: 2673-2680. (IHC, frozen; WB; in vivo tumor targeting) BMC Cancer (2006) 6: 8. (Flow, surface)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4