Pectinase Activity Colorimetric Assay Kit

• Type:

  Colorimetric method

• Field of Research:

  Plant Physiology Series

• Assay Principle:

  Pectinase is a class of pectin decomposition enzymes, including propectinase, pectin esterase, polygalacturonase and pectin lyase, which widely exist in plant fruits and microorganisms, and is the most important enzyme in fruit processing. The detection principle of this kit is that the substance generated by the hydrolysis of pectin by pectinase reacts with the chromogenic agent to produce a brown-red substance with characteristic absorption peak at 540 nm. The activity of pectinase can be calculated by measuring the change of absorbance value at 540 nm.

• Assay Performance Time:

  90 min

• Sample Type:

  Liquid, plant tissue; microorganism

• Detection Range:

  0.05-20 U/mL

• Sensitivity:

  0.05 U/mL

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at 2-8°C for 12 months.

• Inter CV:

  4.5%-6.6%

• Intra CV:

  3.5-4.8%

• Detection Instrument:

  Microplate reader