Glucokinase (GCK) Activity Colorimetric Assay Kit

• Type:

  Colorimetric method

• Field of Research:

  Glycolysis and Lipid Metabolism; Metabolic Diseases

• Assay Principle:

  Glucokinase (GCK) is a key enzyme in the glucose metabolism pathway. It participates in the first step of glycolysis, catalyzes glucose phosphorylation, promotes insulin secretion and glucose metabolism, and effectively controls blood glucose balance in internal. GCK, also known as hexokinase IV, is mainly expressed in hepatocytes and islet β-cell, and has a stronger affinity for glucose than other hexokinases. Therefore, the total hexokinase activity in the sample is measured at high glucose concentration, and the hexokinase activity with low glucose affinity is measured at low glucose concentration. The GCK activity in the sample can be obtained by subtracting the total hexokinase from the total hexokinase. The detection principle of this kit: GCK catalyzes the substrate reaction to produce NADPH, which reacts with the chromogenic agent. The activity of GCK can be calculated by measuring the generation rate of chromogenic substance at 450 nm.

• Assay Performance Time:

  30 min

• Sample Type:

  Animal tissue; Cell

• Detection Range:

  2.62-42.80 U/L

• Sensitivity:

  2.62 U/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Inter CV:

  1.3-7.4%

• Intra CV:

  2.9-6.6%

• Detection Instrument:

  Microplate reader