Anti-Cytokeratin, multi(C11)

• Description:

  This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

• Synonyms:

  K1B; KRT1B; Keratin, type II cytoskeletal 1b; K77; CK-1B; Keratin 1B; Keratin-77; Cytokeratin-1B; Type-II Keratin Kb39

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  KRT4

• Gene ID:

  3851 (CK4); 3852 (CK5); 3853 (CK6A); 3854 (CK6B); 286887 (CK6C); 3856 (CK8); 3858 (CK10); 3860 (CK13); 3875 (CK18)

• UniProt:

  P19013 (CK4); P13647 (CK5); P02538 (CK6A); P04259 (CK6B); P48668 (CK6C); P05787 (CK8); P13645 (CK10); P13646 (CK13); P05783 (CK18)

• Cellular Locus:

  Cytoskeleton

• Host:

  Mouse

• Species Reactivity:

  Cow, Frog, Goat, Guinea pig, Human, Marmoset, Mouse, Pig, Rat

• Immunogen:

  Keratin-enriched preparation from cultured human A431

• Target Antigen:

  Cytokeratin, multi

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  C11

• Conjugation:

  Purified, BSA-free

• Source:

  Animal

• Applications:

  Flow, intracellular (verified) | IF (verified) | IHC, FFPE (verified) | WB (published)

• Validated Applications:

  FC, IF, IHC, FFPE, WB

• Field of Research:

  Cancer, Cytoskeleton

• Positive Control:

  A431 cells, Skin, Colon carcinoma

• Concentration:

  1 mg/mL

• Buffer:

  PBS, no BSA, no azide

• Molecular Weight:

  Multiple

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology formalin-fixed 0.5-1.0ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Immunofluorescence 0.5-1.0 ug/mL|Western blotting 0.5-1.0 ug/mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Clin Cancer Res (2012) 18(4): 993-1003. (IHC, FFPE; western)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4