Anti-Cytokeratin, multi(C11)

Anti-Cytokeratin, multi(C11)

• Description: This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
• Synonyms: K1B; KRT1B; Keratin, type II cytoskeletal 1b; K77; CK-1B; Keratin 1B; Keratin-77; Cytokeratin-1B; Type-II Keratin Kb39
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: KRT4
• Gene ID: 3851 (CK4); 3852 (CK5); 3853 (CK6A); 3854 (CK6B); 286887 (CK6C); 3856 (CK8); 3858 (CK10); 3860 (CK13); 3875 (CK18)
• UniProt: P19013 (CK4); P13647 (CK5); P02538 (CK6A); P04259 (CK6B); P48668 (CK6C); P05787 (CK8); P13645 (CK10); P13646 (CK13); P05783 (CK18)
• Cellular Locus: Cytoskeleton
• Host: Mouse
• Species Reactivity: Cow, Frog, Goat, Guinea pig, Human, Marmoset, Mouse, Pig, Rat
• Immunogen: Keratin-enriched preparation from cultured human A431
• Target Antigen: Cytokeratin, multi
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: C11
• Conjugation: Purified, with BSA
• Source: Animal
• Applications: Flow, intracellular (verified) | IF (verified) | IHC, FFPE (verified) | WB (published)
• Validated Applications: FC, IF, IHC, FFPE, WB
• Field of Research: Cancer, Cytoskeleton
• Positive Control: A431 cells, Skin, Colon carcinoma
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: Multiple
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology formalin-fixed 0.5-1.0ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Immunofluorescence 0.5-1.0 ug/mL|Western blotting 0.5-1.0 ug/mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Clin Cancer Res (2012) 18(4): 993-1003. (IHC, FFPE; western)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years