Sheep C3a (Complement Component 3a) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep C3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep C3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep C3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep C3a in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  Complement Component 3a

• Gene Aliases:

  Complement Component 3a

• Reactivity:

  Sheep

• Field of Research:

  Infection immunity, Immune molecule, Autoimmunity

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep C3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep C3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep C3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep C3a in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  600 ng/mL

• Sample Type:

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range:

  9.38 - 600 ng/mL

• Sensitivity:

  3.73 ng/mL

• Species:

  Sheep

• CAS Number:

  7732-18-5