Dog HSP70 (HeatShock Protein 70) ELISA Kit

Dog HSP70 (HeatShock Protein 70) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog HSP70. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog HSP70. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog HSP70, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog HSP70 in the samples is then determined by comparing the OD of the samples to the standard curve.
• Gene Aliases: DnaK; 70 Kilodalton Heat Shock Proteins;Heat shock 70 kDa protein 1; 2; heat shock 70kDa protein 1A; HSP70; HSP70 1; HSP70 1; HSP70 2; HSP70 1A; HSP70.1; HSP70.2; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B; HSP70; Heat Shock Protein 70
• Field of Research: Tumor immunity; Infection immunity; Developmental science.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 50 ng/mL
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.78-50 ng/mL
• Sensitivity: 0.31 ng/mL