Anti-Cytokeratin 18(KRT18/836), CF594 conjugate

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Anti-Cytokeratin 18(KRT18/836), CF594 conjugate

CAT:

37-BNC940836-500

Size:

500 µL

Anti-Cytokeratin 18(KRT18/836), CF594 conjugate

Description: This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Synonyms: Cell Proliferation-inducing Gene 46 Protein; CK18; CYK18 Cytokeratin Endo B; K18; Keratin-18; Kerd; KRT18
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: KRT18
Gene ID: 3875
NCBI Gene ID: 406013
UniProt: P05783
Cellular Locus: Cytoskeleton
Host: Mouse
Species Reactivity: Cat, Dog, Human
Immunogen: Recombinant human KRT18 protein
Target Antigen: Cytokeratin 18
Clonality: Monoclonal
Isotype: IgG1
Clone: KRT18/836
Conjugation: CF594
Disease: Tumor
Source: Animal
Applications: IF (verified) | IHC, FFPE (verified) | WB (verified)
Validated Applications: IF, IHC, FFPE, WB
Field of Research: Cancer, Cytoskeleton
Positive Control: MCF-7, HeLa, A431 cells or Breast Cancer
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 45 kDa
Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Does not react with rat; others not known|Immunohistology formalin-fixed 2-4 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
Shipping Conditions: Room temperature
Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: 2 years

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