Chicken PCNA (Proliferating Cell Nuclear Antigen) ELISA Kit
CAT:
855-ELK0709-01
Size:
48 Tests
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Chicken PCNA (Proliferating Cell Nuclear Antigen) ELISA Kit
- Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken PCNA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken PCNA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken PCNA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken PCNA in the samples is then determined by comparing the OD of the samples to the standard curve.
- Gene Aliases: Cyclin
- UniProt: Q9DEA3
- Field of Research: Tumor immunity; Developmental science.
- Assay Principle: Sandwich ELISA
- Assay Performance Time: 3.5h
- Standard: 200 ng/mL
- Sample Type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Detection Range: 3.13-200 ng/mL
- Sensitivity: 1.37 ng/mL