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Human PEth (Phosphatidylethanol) ELISA Kit

CAT: 0855-ELK0805-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK0805-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PEth. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PEth. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PEth, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PEth in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
PEth
Reactivity
Human
Field of Research
Cardiovascular biology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PEth. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PEth. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PEth, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PEth in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
500 ng/mL
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
7.82-500 ng/mL
Sensitivity
3.3 ng/mL

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