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Human GSDME (Gasdermin E) ELISA Kit

CAT: 0855-ELK0917-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK0917-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GSDME. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GSDME. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GSDME, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GSDME in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
DFNA5; ICERE-1
UniProt
O60443
Reactivity
Human
Field of Research
Signal transduction
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GSDME. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GSDME. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GSDME, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GSDME in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.062 ng/mL

UniProtKB · O60443

Gasdermin-E

GSDME_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O60443
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
GSDME
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A4D156, B2RAX9, B3KT05, O14590, Q08AQ8, Q9UBV3
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneCytoplasmMembrane

Disease

DeafnessNon-syndromic deafnessTumor suppressor

PTM

Isopeptide bondLipoproteinPalmitateUbl conjugation

Biological process

Necrosis

Domain

TransmembraneTransmembrane beta strand

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