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Human IFNb (Interferon Beta) ELISA Kit

CAT: 0855-ELK1101-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1101-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFNb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IFNb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IFNb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFNb in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
IFNB1; IFN-B; IFB; IFF; IFNB; Interferon Beta 1 Fibroblast; IFN Beta
UniProt
P01574
Reactivity
Human
Field of Research
Cytokine; Tumor immunity; Infection immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFNb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IFNb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IFNb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFNb in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
500 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
7.82-500 pg/mL
Sensitivity
2.8 pg/mL

UniProtKB · P01574

Interferon beta

IFNB_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P01574
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IFNB1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
IFN-beta
EC number
—
Processing
Precursor
Secondary accessions
Q5VWC9
Protein keywords

Technical term

3D-structurePharmaceuticalProteomics identificationReference proteome

Biological process

Antiviral defense

Molecular function

Cytokine

PTM

Disulfide bondGlycoproteinPhosphoprotein

Cellular component

Secreted

Domain

Signal

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