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ELISA Kit for Interferon Beta (IFNb)

CAT: 0338-TRV-054053-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0338-TRV-054053-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Product Name Alternative
IFNB1; IFN-B; IFB; IFF; IFNB; Interferon Beta 1 Fibroblast
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Beta (IFNb) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Beta (IFNb) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Beta (IFNb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Beta (IFNb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
8. Add 50µ L Stop Solution. Read at 450nm immediately.
Assay Performance Time
3H
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
15.6 - 1000 pg/mL
Precision

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Interferon Beta (IFNb) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Interferon Beta (IFNb) were tested on 3 different plates, 8 replicates in each plate.
CV (%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Sensitivity
The minimum detectable dose of this kit is typically less than 5.9pg/mL
Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Specificity

This assay has high sensitivity and excellent specificity for detection of Interferon Beta (IFNb) .
No significant cross-reactivity or interference between Interferon Beta (IFNb) and analogues was observed.

Method
Double-Antibody Sandwich
Organism Species
Rhesus monkey (Simian)

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