Human C3c (Complement C3 Convertase) ELISA Kit

CAT: 0855-ELK1112-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0855-ELK1112-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C3c. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C3c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C3c, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C3c in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
C4b2a
Reactivity
Human
Field of Research
Signal transduction; Infection immunity; Immune molecule
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C3c. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C3c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C3c, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C3c in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
50 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.79-50 ng/mL
Sensitivity
0.3 ng/mL

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