Human C5c (Complement C5 Convertase) ELISA Kit

CAT: 0855-ELK1871-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0855-ELK1871-01Size:48 Tests
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C5c. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C5c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C5c, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C5c in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
C4b2a3b
Reactivity
Human
Field of Research
Enzyme & Kinase; Infection immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C5c. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C5c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C5c, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C5c in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
2000 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
31.25-2000 pg/mL
Sensitivity
15.1 pg/mL

Related Products

CatalogName
ELK2718-01Human C5 (Complement Component 5) ELISA Kit
ELK1112-01Human C3c (Complement C3 Convertase) ELISA Kit
ELK10508-01Mouse C5 (Complement Component 5) ELISA Kit
ELK11466-01Simian C5 (Complement Component 5) ELISA Kit
ELK10678-01Rat C5 (Complement Component 5) ELISA Kit
ELK5556-01Mouse C3c (Complement C3 Convertase) ELISA Kit
ELK8294-01Pig C3c (C3 Convertase) ELISA Kit
ELK4731-01Human ABCC5 (ATP Binding Cassette Transporter C5) ELISA Kit
ELK11061-01Human C1q (Complement 1q) ELISA Kit
ELK3445-01Human PCSK5 (Proprotein Convertase Subtilisin/Kexin Type 5) ELISA Kit

Alternative Products

CatalogName
JL17170-01Human Complement C3 Convertase (C3 Convertase) ELISA Kit
KBH9324GENLISA Human Complement C3 Convertase (C3 CONVERTASE) ELISA
KBH0373GENLISA™ Human Complement C3 Convertase (C3 Convertase) ELISA
EKU03371Human Complement C3 Convertase (C3 Convertase) ELISA Kit
TRV-0006183ELISA Kit for Complement C3 Convertase (C3 Convertase)
MBS048214-01Human Complement C3 Convertase ELISA Kit
MBS105103-01Human Complement C5 Convertase ELISA Kit
EKU10741Mouse Complement C3 Convertase (C3 Convertase) ELISA Kit
EKU10740Pig Complement C3 Convertase (C3 Convertase) ELISA Kit
KLM5260GENLISA™ Mouse C3 Convertase (Complement C3 Convertase ) ELISA

Popular Products