Rat NOS2/iNOS (NitricOxide Synthase 2, Inducible) ELISA Kit

Rat NOS2/iNOS (NitricOxide Synthase 2, Inducible) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NOS2/iNOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NOS2/iNOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NOS2/iNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NOS2/iNOS in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2; NOS2; Nitric Oxide Synthase 2, Inducible
• UniProt: Q06518
• Field of Research: Enzyme & Kinase; Metabolic pathway; Tumor immunity; Cardiovascular biology.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 100 ng/mL
• Sample Type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 1.57-100 ng/mL
• Sensitivity: 0.62 ng/mL