Human PPAR-γ (Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PPAR-γ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PPAR-γ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PPAR-γ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PPAR-γ in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  PPAR-G; PPARG1; PPARG2; NR1C3; Glitazone Receptor; Nuclear Receptor Subfamily 1 Group C Member 3; PPARg; Peroxisome Proliferator Activated Receptor Gamma

• Gene Aliases:

  PPAR-G; PPARG1; PPARG2; NR1C3; Glitazone Receptor; Nuclear Receptor Subfamily 1 Group C Member 3; PPARg; Peroxisome Proliferator Activated Receptor Gamma

• UniProt:

  P37231

• Reactivity:

  Human

• Field of Research:

  Metabolic pathway, Endocrinology, Cardiovascular biology, Developmental science, Bone metabolism

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PPAR-γ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PPAR-γ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PPAR-γ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PPAR-γ in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  10 ng/mL

• Sample Type:

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range:

  0.16 - 10 ng/mL

• Sensitivity:

  0.057 ng/mL

• Species:

  Human

• CAS Number:

  7732-18-5