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Human MK (Midkine) ELISA Kit

CAT: 0855-ELK1713-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1713-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MK in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
MDK; ARAP; NEGF2; Neurite Growth-Promoting Factor 2; Amphiregulin-associated protein; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2
UniProt
P21741
Reactivity
Human
Field of Research
Signal transduction; Tumor immunity; Developmental science
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MK in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.054 ng/mL

UniProtKB · P21741

Midkine

MK_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P21741
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MDK
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
MK
EC number
—
Processing
Precursor
Secondary accessions
Q2LEK4, Q9UCC7
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Molecular function

Developmental proteinGrowth factorHeparin-bindingMitogen

Biological process

Differentiation

PTM

Disulfide bond

Cellular component

Secreted

Domain

Signal

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