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Mouse VTN (Vitronectin) ELISA Kit

CAT: 0855-ELK11130-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK11130-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VTN in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
V75; VN; VNT; Serum Spreading Factor; Somatomedin B; Complement S-Protein
UniProt
P29788
Reactivity
Mouse
Field of Research
Signal transduction; Metabolic pathway; Tumor immunity; Hepatology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VTN in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
200 μg/mL
Sample Type
Plasma.
Detection Range
3.13-200 μg/mL
Sensitivity
0.99 μg/mL

UniProtKB · P29788

Vitronectin

VTNC_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P29788
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Vtn
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
VN
EC number
—
Processing
Precursor
Secondary accessions
Q5SYG4, Q8VII4, Q91X32, Q9D080
Protein keywords

Biological process

Cell adhesion

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoproteinPhosphoproteinSulfation

Molecular function

Heparin-binding

Domain

RepeatSignal

Cellular component

Secreted

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