Mouse GHRL (Ghrelin) ELISA Kit

CAT: 0855-ELK1945-01Size: 48 TestsDry Ice: NoHazardous: No

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CAT#:0855-ELK1945-01Size:48 Tests

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse GHRL protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GHRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GHRL in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Name Alternative

MTLRP; Growth Hormone-Releasing Peptide

UniProt

Q9UBU5

Reactivity

Mouse

Field of Research

Signal transduction; Metabolic pathway; Endocrinology; Gastroenterology; Hormone metabolism

Assay Type

Competitive Inhibition

Assay Principle

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse GHRL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GHRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GHRL in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

Standard

10000 pg/mL

Sample Type

Serum, plasma, cell lysates, cell culture supernates and other biological fluids

Detection Range

156.25-10000 pg/mL

Sensitivity

45.6 pg/mL

UniProtKB · P30044

Peroxiredoxin-5, mitochondrial

PRDX5_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P30044
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: PRDX5
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: 1.11.1.24
Processing: Precursor
Secondary accessions: A6NC19, A6NG06, B7ZLJ4, B7ZVW3, Q14CK0, Q6IAF2, Q9UBU5, Q9UJU4, Q9UKX4

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationDisulfide bondLipoproteinPalmitatePhosphoprotein

Coding sequence diversity

Alternative initiationAlternative splicing

Molecular function

AntioxidantOxidoreductasePeroxidase

Cellular component

CytoplasmMitochondrionPeroxisome

Domain

Redox-active centerTransit peptide

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