Products for Research Use Only
RegisterLogin

Chicken FGF2 (Fibroblast Growth Factor 2, Basic) ELISA Kit

CAT: 0855-ELK2036-01Size: 48 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0855-ELK2036-01Size:48 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken FGF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken FGF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken FGF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken FGF2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2
UniProt
P48800
Reactivity
Chicken
Field of Research
Cytokine; Tumor immunity; Infection immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken FGF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken FGF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken FGF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken FGF2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 pg/mL
Sample Type
Serum, plasma and other biological fluids.
Detection Range
15.63-1000 pg/mL
Sensitivity
6.4 pg/mL

UniProtKB · P48800

Fibroblast growth factor 2

FGF2_CHICK · Gallus gallus

View on UniProt ↗
Primary accession
P48800
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
FGF2
Protein existence
2: Evidence at transcript level
Organism
Gallus gallus (Chicken)
Taxonomy ID
9031
Alternative names
FGF-2
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

AngiogenesisDifferentiation

Molecular function

Developmental proteinGrowth factorHeparin-bindingMitogen

Cellular component

NucleusSecreted

Technical term

Reference proteome

Related Products

CatalogName
ELK5578-01Rat FGF2 (Fibroblast Growth Factor 2, Basic) ELISA Kit
ELK1258-01Human FGF2 (Fibroblast Growth Factor 2, Basic) ELISA Kit
ELK5579ELISA kit for Human FGF2 (Fibroblast Growth Factor 2, Basic)
ELK4942-01Mouse FGF2 (Fibroblast Growth Factor 2, Basic) ELISA Kit
ELK7261-01Pig FGF2 (Fibroblast Growth Factor 2, Basic) ELISA Kit
ELK5580-01Mouse FGF2 (Fibroblast Growth Factor 2, Basic) ELISA Kit
ELK8555-01Dog FGF2 (Fibroblast Growth Factor 2, Basic) ELISA Kit
ELK2598ELISA kit for Rat FGF2 (Fibroblast Growth Factor 2, Basic)
ELK7546-01Cattle FGF2 (Fibroblast Growth Factor 2, Basic) ELISA Kit
ELK2429-01Rabbit FGF2 (Fibroblast Growth Factor 2, Basic) ELISA Kit

Alternative Products

CatalogName
GWB-A036E8Fibroblast Growth Factor-basic (FGF-Basic)
RC215-13FGF-basic, Fibroblast Growth Factor-basic, human
216530Fibroblast Growth Factor, Basic (FGFb, FGF basic)
GTR18793469Basic Fibroblast growth factor (Basic FGF) Antibody
RC265-13FGF-basic, Fibroblast Growth Factor-basic, bovine
RC255-13FGF-basic, Fibroblast Growth Factor-basic, rat
MBS735316-01Chicken Basic Fibroblast Growth Factor ELISA Kit
MBS761331-01Mouse Basic Fibroblast Growth Factor ELISA Kit
MBS743948-01Canine Basic Fibroblast Growth Factor ELISA Kit
GTR10462944Rabbit Basic Fibroblast growth factor ELISA kit

Popular Products