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Human OIT3 (Oncoprotein Induced Transcript 3) ELISA Kit

CAT: 0855-ELK2163-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2163-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OIT3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OIT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OIT3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OIT3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
LZP; Liver-specific zona pellucida domain-containing protein
UniProt
Q8WWZ8
Reactivity
Human
Field of Research
Signal transduction; Tumor immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OIT3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OIT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OIT3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OIT3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.32-20 ng/mL
Sensitivity
0.113 ng/mL

UniProtKB · Q8WWZ8

Oncoprotein-induced transcript 3 protein

OIT3_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q8WWZ8
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
OIT3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A0AVP3, Q8N1M8
Protein keywords

Coding sequence diversity

Alternative splicing

Ligand

Calcium

PTM

Disulfide bondGlycoprotein

Domain

EGF-like domainSignal

Cellular component

Nucleus

Technical term

Proteomics identificationReference proteome

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