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Mouse DDIT3 (DNA Damage Inducible Transcript 3) ELISA Kit

CAT: 0855-ELK7029-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK7029-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DDIT3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse DDIT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse DDIT3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse DDIT3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CEBPZ; CHOP; CHOP10; GADD153; C/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; Growth arrest and DNA damage-inducible GADD153
UniProt
P35639
Reactivity
Mouse
Field of Research
Signal transduction
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DDIT3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse DDIT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse DDIT3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse DDIT3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.054 ng/mL

UniProtKB · P35639

DNA damage-inducible transcript 3 protein

DDIT3_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P35639
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ddit3
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
DDIT-3
EC number
—
Processing
—
Secondary accessions
Q91YW9
Protein keywords

Molecular function

ActivatorDNA-bindingRepressor

Coding sequence diversity

Alternative initiation

Biological process

ApoptosisCell cycleGrowth arrestStress responseTranscriptionTranscription regulationUnfolded protein responseWnt signaling pathway

Cellular component

CytoplasmNucleus

PTM

PhosphoproteinUbl conjugation

Technical term

Reference proteome

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