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Human XPC (Xeroderma Pigmentosum, Complementation Group C) ELISA Kit

CAT: 0855-ELK2394-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2394-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human XPC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human XPC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human XPC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human XPC in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
XP-C; XP3; XPCC; RAD4; Xeroderma Pigmentosum Group C Protein; DNA repair protein complementing XP-C cells
UniProt
Q01831
Reactivity
Human
Field of Research
Metabolic pathway; Tumor immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human XPC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human XPC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human XPC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human XPC in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.11 ng/mL

UniProtKB · Q01831

DNA repair protein complementing XP-C cells

XPC_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q01831
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
XPC
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B4DIP3, E9PB96, E9PH69, Q53GT7, Q96AX0
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

ChromosomeCytoplasmNucleus

Disease

Disease variantXeroderma pigmentosum

Biological process

DNA damageDNA repairTranscriptionTranscription regulation

Molecular function

DNA-binding

PTM

Isopeptide bondPhosphoproteinUbl conjugation

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