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Human XPD (Xeroderma Pigmentosum, Complementation Group D) ELISA Kit

CAT: 0855-ELK5232-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK5232-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human XPD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human XPD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human XPD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human XPD in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CXPD; BTF2 p80; EM9; TTD; ERCC2; TFIIH basal transcription factor complex 80 kDa; De Sanctis-Cacchione; Excision Repair Cross-Complementing Rodent Repair Deficiency 2
UniProt
P18074
Reactivity
Human
Field of Research
Signal transduction; Tumor immunity; Dermatology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human XPD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human XPD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human XPD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human XPD in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.059 ng/mL

UniProtKB · P18074

General transcription and DNA repair factor IIH helicase subunit XPD

ERCC2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P18074
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ERCC2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
TFIIH subunit XPD
EC number
5.6.2.3
Processing
—
Secondary accessions
Q2TB78, Q2YDY2, Q7KZU6, Q8N721
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Ligand

4Fe-4SATP-bindingIronIron-sulfurMagnesiumMetal-bindingNucleotide-binding

Coding sequence diversity

Alternative splicing

Disease

CataractCockayne syndromeDeafnessDisease variantDwarfismIchthyosisXeroderma pigmentosum

Biological process

Chromosome partitionDNA damageDNA repairHost-virus interactionTranscriptionTranscription regulation

Cellular component

CytoplasmCytoskeletonNucleus

Molecular function

DNA-bindingHelicaseHydrolaseIsomerase

PTM

Ubl conjugation

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