Rat NT-ProANP (N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit
CAT:
855-ELK2400-02
Size:
96 Tests
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Rat NT-ProANP (N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit
- Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NT-ProANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NT-ProANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NT-ProANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NT-ProANP in the samples is then determined by comparing the OD of the samples to the standard curve.
- CAS Number: 9000-83-3
- Gene Aliases: NT-Pro-ANP; N-ANP
- Field of Research: Endocrinology; Cardiovascular biology.
- Assay Principle: Sandwich ELISA
- Assay Performance Time: 3.5h
- Standard: 40 ng/mL
- Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
- Detection Range: 0.63-40 ng/mL
- Sensitivity: 0.258 ng/mL
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