Human NT-ProANP (N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit

Human NT-ProANP (N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT-ProANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NT-ProANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProANP in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 9000-83-3
• Gene Aliases: NT-Pro-ANP; N-ANP
• Field of Research: Endocrinology; Cardiovascular biology.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 50 ng/mL
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.79-50 ng/mL
• Sensitivity: 0.31 ng/mL
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