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Mouse Des (Desmin) ELISA Kit

CAT: 0855-ELK2656-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2656-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Des. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Des. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Des, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Des in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CMD1I; CSM1; CSM2; Intermediate Filament Protein
UniProt
P31001
Reactivity
Mouse
Field of Research
Signal transduction; Metabolic pathway; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Des. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Des. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Des, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Des in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.045 ng/mL

UniProtKB · P31001

Desmin

DESM_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P31001
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Des
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

PTM

ADP-ribosylationMethylationPhosphoproteinUbl conjugation

Cellular component

Cell membraneCytoplasmIntermediate filamentMembraneNucleus

Domain

Coiled coil

Molecular function

Muscle protein

Technical term

Reference proteome

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