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Mouse VCL (Vinculin) ELISA Kit

CAT: 0855-ELK2781-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2781-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VCL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VCL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VCL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VCL in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
MVCL; Metavinculin
UniProt
Q64727
Reactivity
Mouse
Field of Research
Metabolic pathway; Developmental science; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VCL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VCL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VCL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VCL in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.132 ng/mL

UniProtKB · Q64727

Vinculin

VINC_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q64727
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Vcl
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q8BP32, Q8BS46, Q922C5, Q922D9
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

PTM

AcetylationLipoproteinPalmitatePhosphoprotein

Molecular function

Actin-binding

Biological process

Cell adhesion

Cellular component

Cell junctionCell membraneCell projectionCytoplasmCytoskeletonMembrane

Domain

Repeat

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