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Human GAL8 (Galectin 8) ELISA Kit

CAT: 0855-ELK2896-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2896-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GAL8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GAL8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GAL8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GAL8 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
LGALS8; PCTA1; Po66-CBP; Prostate Carcinoma Tumor Antigen 1; Lectin, Galactoside-Binding Soluble 8; Po66 carbohydrate-binding protein; Prostate carcinoma tumor antigen 1
UniProt
O00214
Reactivity
Human
Field of Research
Tumor immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GAL8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GAL8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GAL8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GAL8 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.061 ng/mL

UniProtKB · O00214

Galectin-8

LEG8_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O00214
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
LGALS8
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
Gal-8
EC number
—
Processing
—
Secondary accessions
O15215, Q5T3P5, Q5T3Q4, Q8TEV1, Q96B92, Q9BXC8, Q9H584, Q9H585, Q9UEZ6, Q9UP32, Q9UP33, Q9UP34
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

Autophagy

Cellular component

CytoplasmCytoplasmic vesicle

Ligand

Lectin

Domain

Repeat

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