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Human CPE (Carboxypeptidase E) ELISA Kit

CAT: 0855-ELK3132-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3132-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CPE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CPE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CPE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CPE in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CPH; Enkephalin Convertase; Carboxypeptidase H; Prohormone-processing carboxypeptidase
UniProt
P16870
Reactivity
Human
Field of Research
Enzyme & Kinase
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CPE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CPE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CPE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CPE in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
40 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.63-40 ng/mL
Sensitivity
0.242 ng/mL

UniProtKB · P16870

Carboxypeptidase E

CBPE_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P16870
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CPE
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
CPE
EC number
3.4.17.10
Processing
Precursor
Secondary accessions
A8K4N1, B3KR42, B4DFN4, D3DP33, Q9UIU9
Protein keywords

Coding sequence diversity

Alternative splicing

Molecular function

CarboxypeptidaseHydrolaseMetalloproteaseProtease

PTM

Cleavage on pair of basic residuesGlycoproteinZymogen

Cellular component

Cytoplasmic vesicleMembraneSecreted

Disease

Disease variantHypogonadotropic hypogonadismIntellectual disabilityObesity

Ligand

Metal-bindingZinc

Technical term

Proteomics identificationReference proteome

Domain

Signal

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