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Human C1s (Complement Component 1, S Subcomponent) ELISA Kit

CAT: 0855-ELK3175-03Size: 5x 96 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3175-03Size:5x 96 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C1s. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C1s. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C1s, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C1s in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
C1 esterase
UniProt
P09871
Reactivity
Human
Field of Research
Signal transduction; Infection immunity; Immune molecule
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C1s. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C1s. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C1s, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C1s in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
200 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
3.13-200 ng/mL
Sensitivity
1.24 ng/mL

UniProtKB · P09871

Complement C1s subcomponent

C1S_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P09871
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
C1S
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.4.21.42
Processing
Precursor
Secondary accessions
D3DUT4, Q9UCU7, Q9UCU8, Q9UCU9, Q9UCV0, Q9UCV1, Q9UCV2, Q9UCV3, Q9UCV4, Q9UCV5, Q9UM14
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Ligand

CalciumMetal-binding

Biological process

Complement pathwayImmunityInnate immunity

Disease

Disease variantEhlers-Danlos syndrome

PTM

Disulfide bondGlycoproteinHydroxylation

Domain

EGF-like domainRepeatSignalSushi

Molecular function

HydrolaseProteaseSerine protease

Cellular component

Secreted

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