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Human GLRX3 (Glutaredoxin 3) ELISA Kit

CAT: 0855-ELK3407-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3407-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GLRX3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GLRX3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GLRX3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GLRX3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
TXNL2; PICOT; GRX3; GLRX4; GRX4; Thioredoxin-Like 2; PKC-interacting cousin of thioredoxin; PKC-theta-interacting protein; Thioredoxin-like protein 2
UniProt
O76003
Reactivity
Human
Field of Research
Enzyme & Kinase
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GLRX3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GLRX3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GLRX3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GLRX3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Plasma
Detection Range
0.16-10 ng/mL
Sensitivity
0.07 ng/mL

UniProtKB · O76003

Glutaredoxin-3

GLRX3_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O76003
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
GLRX3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B3KMP7, B3KMQ5, D3DRG2, Q5JV01, Q96CE0, Q9P1B0, Q9P1B1
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Cellular component

Cytoplasm

Ligand

IronIron-sulfurMetal-binding

Domain

Repeat

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