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Human DYSF (Dysferlin) ELISA Kit

CAT: 0855-ELK3572-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3572-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DYSF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DYSF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DYSF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DYSF in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
FER1L1; LGMD2B; Dysferlin, Limb Girdle Muscular Dystrophy 2B (Autosomal Recessive) ; Fer-1-like protein 1; Dystrophy-associated fer-1-like protein
UniProt
O75923
Reactivity
Human
Field of Research
Immune molecule; Neuro science; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DYSF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DYSF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DYSF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DYSF in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
2000 pg/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
31.25-2000 pg/mL
Sensitivity
12.7 pg/mL

UniProtKB · O75923

Dysferlin

DYSF_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O75923
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
DYSF
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A0FK00, B1PZ70, B1PZ71, B1PZ72, B1PZ73, B1PZ74, B1PZ75, B1PZ76, B1PZ77, B1PZ78, B1PZ79, B1PZ80, B1PZ81, B3KQB9, O75696, Q09EX5, Q0H395, Q53QY3, Q53TD2, Q8TEL8, Q9UEN7
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative promoter usageAlternative splicing

Ligand

CalciumLipid-bindingMetal-binding

Cellular component

Cell membraneCytoplasmic vesicleEndosomeMembrane

Disease

Disease variantLimb-girdle muscular dystrophy

PTM

Phosphoprotein

Domain

RepeatSignal-anchorTransmembraneTransmembrane helix

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