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Human PLD2 (Phospholipase D2) ELISA Kit

CAT: 0855-ELK4093-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4093-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PLD2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PLD2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PLD2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PLD2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
PLD1C; Choline Phosphatase 2; Phosphatidylcholine-hydrolyzing phospholipase D2
UniProt
O14939
Reactivity
Human
Field of Research
Metabolic pathway
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PLD2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PLD2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PLD2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PLD2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
100 ng/mL
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
1.57-100 ng/mL
Sensitivity
0.55 ng/mL

UniProtKB · O14939

Phospholipase D2

PLD2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O14939
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PLD2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
PLD 2, hPLD2
EC number
3.1.4.4
Processing
—
Secondary accessions
I3L2C9, O43540, O43579, O43580, Q6PGR0, Q96BY3
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembrane

Molecular function

Hydrolase

Biological process

Lipid degradationLipid metabolism

PTM

LipoproteinPhosphoprotein

Domain

Repeat

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